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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- MA5-38164 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG10 Recombinant Rabbit Monoclonal Antibody (ARC1425)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: HeLa, 293T, K-562, SH-SY5Y, Mouse heart Immunogen sequence: RPLTLKDIWE GVHECYKMRL LQGPWDTITQ QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ KINKNVNYIT SWLSIVGPVV GLNLPLSYAK ATSQDERNVP
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- ARC1425
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG10 using ATG10 Recombinant Monoclonal Antibody (Product # MA5-38164) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of ATG10 in NIH-3T3 cells using ATG10 Recombinant Monoclonal Antibody (Product # MA5-38164) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of ATG10 in NIH-3T3 cells using ATG10 Recombinant Monoclonal Antibody (Product # MA5-38164) at a dilution of 1:100. Blue: DAPI for nuclear staining.
