MA5-38164

antibody from Invitrogen Antibodies

Targeting: ATG10 APG10L, DKFZP586I0418, FLJ13954

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38164

Provider

Invitrogen Antibodies

Product name

ATG10 Recombinant Rabbit Monoclonal Antibody (2Q3Q3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Samples: HeLa, 293T, K-562, SH-SY5Y, Mouse heart Immunogen sequence: RPLTLKDIWE GVHECYKMRL LQGPWDTITQ QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ KINKNVNYIT SWLSIVGPVV GLNLPLSYAK ATSQDERNVP

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

2Q3Q3

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATG10 in extracts of various cell lines. Samples were incubated with ATG10 Monoclonal antibody (Product # MA5-38164) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of ATG10 in NIH-3T3 cells using ATG10 Recombinant Monoclonal Antibody (Product # MA5-38164) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of ATG10 in NIH-3T3 cells using ATG10 Recombinant Monoclonal Antibody (Product # MA5-38164) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ATG10 in NIH-3T3 cells. Samples were incubated with ATG10 Monoclonal antibody (Product # MA5-38164) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.