Antibody data
- Antibody Data
- Antigen structure
- References [36]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 13859-1-AP - Provider product page

- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13859-1-AP, RRID:AB_2204679
- Product name
- TIMM44 antibody
- Antibody type
- Polyclonal
- Description
- TIMM44 antibody (Cat. #13859-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A dynamic gateway: Uncovering the expanded human TOM complex interactome and its regulatory complexity.
Magnetic Metal-Organic Framework (MOF)-Mediated Precision Capture of Mitochondria Reveals Subcellular Metabolic and Lipid Remodeling of Crabtree Effect in Yeast.
Stress-induced OMA1-mediated cleavage of AIFM1 suppresses cell growth by controlling mitochondrial OXPHOS activity.
A unified mechanism for mitochondrial damage sensing in PINK1-Parkin-mediated mitophagy.
DDX49 as a novel prognostic biomarker regulates colorectal cancer cell proliferation through the TIMM44-PI3K-AKT pathway.
C19orf12 inhibits mitochondrial function and enhances the antitumor effects of metformin in non-small cell lung cancer.
Legionella effector LpPIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins.
Novel reporter of the PINK1-Parkin mitophagy pathway identifies its damage sensor in the import gate.
Multimodal analysis stratifies genetic susceptibility and reveals the pathogenic mechanism of kidney injury in diabetic nephropathy.
The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve.
In vitro study on the promotion of osteogenic differentiation by mitochondrial-derived vesicles through activation of inflammation and reprogramming of metabolic pathways.
STED super-resolution microscopy of mitochondrial translocases.
Mycobacterial CpsA activates type I IFN signaling in macrophages via cGAS-mediated pathway.
CLPB disaggregase dysfunction impacts the functional integrity of the proteolytic SPY complex.
OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase.
Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologies.
Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria.
LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transport.
TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria.
ATAXIA TELANGIECTASIA MUTATED PROTECTS AGAINST LIPOPOLYSACCARIDE-INDUCED BLOOD-BRAIN BARRIER DISRUPTION BY REGULATING ATK/DRP1-MEDIATED MITOCHONDRIAL HOMEOSTASIS.
Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy.
Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.
Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.
ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.
Super-resolution microscopy reveals the arrangement of inner membrane protein complexes in mammalian mitochondria.
LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.
Balancing of mitochondrial translation through METTL8-mediated m(3)C modification of mitochondrial tRNAs.
Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.
The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.
PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.
Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
Translocase of inner mitochondrial membrane 44 alters the mitochondrial fusion and fission dynamics and protects from type 2 diabetes.
Borrero-Landazabal MA, Linke V, Kadavá T, Li Z, Draczkowski P, Kaszuba K, Bertgen L, Serwa RA, Heck AJR, Chacinska A
Science advances 2026 May;12(18):eaeb2995
Science advances 2026 May;12(18):eaeb2995
Magnetic Metal-Organic Framework (MOF)-Mediated Precision Capture of Mitochondria Reveals Subcellular Metabolic and Lipid Remodeling of Crabtree Effect in Yeast.
Du T, Li T, Chen F, Huang D, Han R, Zhao C, Liu X, Shi X, Xu G
Analytical chemistry 2026 May 19;98(19):14086-14095
Analytical chemistry 2026 May 19;98(19):14086-14095
Stress-induced OMA1-mediated cleavage of AIFM1 suppresses cell growth by controlling mitochondrial OXPHOS activity.
Nishigori M, Hirata S, Kosako H, Ichinohe T, Nolte H, Riemer J, Langer T, Koshiba T
The EMBO journal 2026 Mar 24;
The EMBO journal 2026 Mar 24;
A unified mechanism for mitochondrial damage sensing in PINK1-Parkin-mediated mitophagy.
Thayer JA, Petersen JD, Huang X, Gruel Budet LM, Hawrot J, Ramos DM, Sekine S, Li Y, Ward ME, Narendra DP
The EMBO journal 2026 Jan;45(1):64-105
The EMBO journal 2026 Jan;45(1):64-105
DDX49 as a novel prognostic biomarker regulates colorectal cancer cell proliferation through the TIMM44-PI3K-AKT pathway.
Huang B, Yin Z, Gao G, Li L, Yang M
Frontiers in oncology 2025;15:1707092
Frontiers in oncology 2025;15:1707092
C19orf12 inhibits mitochondrial function and enhances the antitumor effects of metformin in non-small cell lung cancer.
Liu R, Zhao M, Zhang X, Wu H, Ren L, Lv J, Liu Z, Wang Q, Yao Z
Cell reports 2025 Sep 23;44(9):116163
Cell reports 2025 Sep 23;44(9):116163
Legionella effector LpPIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins.
Yek KQ, Hodgson ER, Ang CS, Palmer CS, Frazier AE, Newton HJ, Stojanovski D
PLoS biology 2025 Jul;23(7):e3003261
PLoS biology 2025 Jul;23(7):e3003261
Novel reporter of the PINK1-Parkin mitophagy pathway identifies its damage sensor in the import gate.
Thayer JA, Petersen JD, Huang X, Hawrot J, Ramos DM, Sekine S, Li Y, Ward ME, Narendra DP
bioRxiv : the preprint server for biology 2025 Feb 20;
bioRxiv : the preprint server for biology 2025 Feb 20;
Multimodal analysis stratifies genetic susceptibility and reveals the pathogenic mechanism of kidney injury in diabetic nephropathy.
Jiang S, Jia H, Hou Q, Jin L, Ahsan MA, Li G, Guan T, Zhao J, Liu Z, Xie J, Cheng H, Hao C, Wan J, Ni Z, Wang N, Shi J, Zheng C, Zhang R, Yan D, Chen H, Jia W, Shen N, Hu C, Liu Z
Cell reports. Medicine 2025 Aug 19;6(8):102249
Cell reports. Medicine 2025 Aug 19;6(8):102249
The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve.
Ma Q, Wu L, Wu J, Ni B, Wang J, Song S
Reproductive sciences (Thousand Oaks, Calif.) 2025 Apr;32(4):1202-1212
Reproductive sciences (Thousand Oaks, Calif.) 2025 Apr;32(4):1202-1212
In vitro study on the promotion of osteogenic differentiation by mitochondrial-derived vesicles through activation of inflammation and reprogramming of metabolic pathways.
Pan C, Cheng C, Zhong S, Li S, Tan W, Yao Y
Journal of orthopaedic surgery and research 2025 Apr 17;20(1):388
Journal of orthopaedic surgery and research 2025 Apr 17;20(1):388
STED super-resolution microscopy of mitochondrial translocases.
Schweighofer SV, Inamdar K, Jans DC, Jakobs S
Methods in enzymology 2024;707:299-327
Methods in enzymology 2024;707:299-327
Mycobacterial CpsA activates type I IFN signaling in macrophages via cGAS-mediated pathway.
Ding Y, Tong J, Luo G, Sun R, Bei C, Feng Z, Meng L, Wang F, Zhou J, Chen Z, Li D, Fan Y, Song S, Wang D, Feng CG, Liu H, Chen Q, Yan B, Gao Q
iScience 2024 May 17;27(5):109807
iScience 2024 May 17;27(5):109807
CLPB disaggregase dysfunction impacts the functional integrity of the proteolytic SPY complex.
Baker MJ, Blau KU, Anderson AJ, Palmer CS, Fielden LF, Crameri JJ, Milenkovic D, Thorburn DR, Frazier AE, Langer T, Stojanovski D
The Journal of cell biology 2024 Mar 4;223(3)
The Journal of cell biology 2024 Mar 4;223(3)
OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase.
Elancheliyan P, Maruszczak KK, Serwa RA, Stephan T, Gulgec AS, Borrero-Landazabal MA, Ngati S, Gosk A, Jakobs S, Wasilewski M, Chacinska A
Cell reports 2024 Dec 24;43(12):115038
Cell reports 2024 Dec 24;43(12):115038
Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologies.
Zhu Y, Akkaya KC, Ruta J, Yokoyama N, Wang C, Ruwolt M, Lima DB, Lehmann M, Liu F
Nature communications 2024 Apr 17;15(1):3290
Nature communications 2024 Apr 17;15(1):3290
Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria.
Speck SL, Bhatt DP, Zhang Q, Adak S, Yin L, Dong G, Feng C, Zhang W, Ben Major M, Wei X, Semenkovich CF
Cell reports 2023 Nov 28;42(11):113389
Cell reports 2023 Nov 28;42(11):113389
LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transport.
Sun H, Zhang J, Ye Q, Jiang T, Liu X, Zhang X, Zeng F, Li J, Zheng Y, Han X, Su C, Shi Y
Cell reports 2023 Nov 28;42(11):113214
Cell reports 2023 Nov 28;42(11):113214
TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria.
Akabane S, Watanabe K, Kosako H, Yamashita SI, Nishino K, Kato M, Sekine S, Kanki T, Matsuda N, Endo T, Oka T
Cell reports 2023 May 30;42(5):112454
Cell reports 2023 May 30;42(5):112454
ATAXIA TELANGIECTASIA MUTATED PROTECTS AGAINST LIPOPOLYSACCARIDE-INDUCED BLOOD-BRAIN BARRIER DISRUPTION BY REGULATING ATK/DRP1-MEDIATED MITOCHONDRIAL HOMEOSTASIS.
Luo S, Lyu Z, Ge L, Li Y, Liu Y, Yuan Y, Zhao R, Huang L, Zhao J, Huang H, Luo Y
Shock (Augusta, Ga.) 2023 Jul 1;60(1):100-109
Shock (Augusta, Ga.) 2023 Jul 1;60(1):100-109
Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy.
Michaelis JB, Brunstein ME, Bozkurt S, Alves L, Wegner M, Kaulich M, Pohl C, Münch C
Nature communications 2022 Sep 2;13(1):5164
Nature communications 2022 Sep 2;13(1):5164
Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.
Mao X, Gu Y, Sui X, Shen L, Han J, Wang H, Xi Q, Zhuang Q, Meng Q, Wu G
Frontiers in cell and developmental biology 2021;9:673618
Frontiers in cell and developmental biology 2021;9:673618
Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.
Fielden LF, Scott NE, Palmer CS, Khoo CA, Newton HJ, Stojanovski D
Molecular & cellular proteomics : MCP 2021;20:100005
Molecular & cellular proteomics : MCP 2021;20:100005
ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.
Lin BC, Phung TH, Higgins NR, Greenslade JE, Prado MA, Finley D, Karbowski M, Polster BM, Monteiro MJ
Human molecular genetics 2021 Jun 17;30(13):1230-1246
Human molecular genetics 2021 Jun 17;30(13):1230-1246
Super-resolution microscopy reveals the arrangement of inner membrane protein complexes in mammalian mitochondria.
Palmer CS, Lou J, Kouskousis B, Pandzic E, Anderson AJ, Kang Y, Hinde E, Stojanovski D
Journal of cell science 2021 Jul 1;134(13)
Journal of cell science 2021 Jul 1;134(13)
LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.
Shin CS, Meng S, Garbis SD, Moradian A, Taylor RW, Sweredoski MJ, Lomenick B, Chan DC
Nature communications 2021 Jan 11;12(1):265
Nature communications 2021 Jan 11;12(1):265
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Morgenstern M, Peikert CD, Lübbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, Mühlhäuser WWD, Knapp B, Busch JD, Stiller SB, Dannenmaier S, Lindau C, Licheva M, Eickhorst C, Galbusera R, Zerbes RM, Ryan MT, Kraft C, Kozjak-Pavlovic V, Drepper F, Dennerlein S, Oeljeklaus S, Pfanner N, Wiedemann N, Warscheid B
Cell metabolism 2021 Dec 7;33(12):2464-2483.e18
Cell metabolism 2021 Dec 7;33(12):2464-2483.e18
Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.
Dennerlein S, Poerschke S, Oeljeklaus S, Wang C, Richter-Dennerlein R, Sattmann J, Bauermeister D, Hanitsch E, Stoldt S, Langer T, Jakobs S, Warscheid B, Rehling P
eLife 2021 Dec 31;10
eLife 2021 Dec 31;10
Balancing of mitochondrial translation through METTL8-mediated m(3)C modification of mitochondrial tRNAs.
Schöller E, Marks J, Marchand V, Bruckmann A, Powell CA, Reichold M, Mutti CD, Dettmer K, Feederle R, Hüttelmaier S, Helm M, Oefner P, Minczuk M, Motorin Y, Hafner M, Meister G
Molecular cell 2021 Dec 2;81(23):4810-4825.e12
Molecular cell 2021 Dec 2;81(23):4810-4825.e12
Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.
Kang Y, Anderson AJ, Jackson TD, Palmer CS, De Souza DP, Fujihara KM, Stait T, Frazier AE, Clemons NJ, Tull D, Thorburn DR, McConville MJ, Ryan MT, Stroud DA, Stojanovski D
eLife 2019 Nov 4;8
eLife 2019 Nov 4;8
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
Lavdovskaia E, Kolander E, Steube E, Mai MM, Urlaub H, Richter-Dennerlein R
Nucleic acids research 2018 Sep 19;46(16):8471-8482
Nucleic acids research 2018 Sep 19;46(16):8471-8482
COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.
Aich A, Wang C, Chowdhury A, Ronsör C, Pacheu-Grau D, Richter-Dennerlein R, Dennerlein S, Rehling P
eLife 2018 Jan 30;7
eLife 2018 Jan 30;7
The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.
Rauschenberger V, Bernkopf DB, Krenn S, Jalal K, Heller J, Behrens J, Gentzel M, Schambony A
Development (Cambridge, England) 2017 Jun 15;144(12):2234-2247
Development (Cambridge, England) 2017 Jun 15;144(12):2234-2247
PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.
Saita S, Nolte H, Fiedler KU, Kashkar H, Venne AS, Zahedi RP, Krüger M, Langer T
Nature cell biology 2017 Apr;19(4):318-328
Nature cell biology 2017 Apr;19(4):318-328
Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
Richter-Dennerlein R, Oeljeklaus S, Lorenzi I, Ronsör C, Bareth B, Schendzielorz AB, Wang C, Warscheid B, Rehling P, Dennerlein S
Cell 2016 Oct 6;167(2):471-483.e10
Cell 2016 Oct 6;167(2):471-483.e10
Translocase of inner mitochondrial membrane 44 alters the mitochondrial fusion and fission dynamics and protects from type 2 diabetes.
Wang Y, Katayama A, Terami T, Han X, Nunoue T, Zhang D, Teshigawara S, Eguchi J, Nakatsuka A, Murakami K, Ogawa D, Furuta Y, Makino H, Wada J
Metabolism: clinical and experimental 2015 Jun;64(6):677-88
Metabolism: clinical and experimental 2015 Jun;64(6):677-88
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13859-1-AP(TIMM44 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Immunofluorescent analysis of A549 cells, using TIMM44 antibody 13859-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line