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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [3]
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- Product number
- HPA043052 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA043052, RRID:AB_10795713
- Product name
- Anti-TIMM44
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TIMM44, Gene description: translocase of inner mitochondrial membrane 44 homolog (yeast), Alternative Gene Names: TIM44, Validated applications: WB, IHC, ICC, Uniprot ID: O43615, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization
Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis
TDP-43 Triggers Mitochondrial DNA Release via mPTP to Activate cGAS/STING in ALS.
VDAC2 enables BAX to mediate apoptosis and limit tumor development
Autophagy induced during apoptosis degrades mitochondria and inhibits type I interferon secretion
Desmurs M, Foti M, Raemy E, Vaz F, Martinou J, Bairoch A, Lane L
Molecular and Cellular Biology 2023;35(7):1139-1156
Molecular and Cellular Biology 2023;35(7):1139-1156
Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis
Carter R, Milani M, Beckett A, Liu S, Prior I, Cohen G, Varadarajan S
Cell Death & Disease 2022;13(5)
Cell Death & Disease 2022;13(5)
TDP-43 Triggers Mitochondrial DNA Release via mPTP to Activate cGAS/STING in ALS.
Yu CH, Davidson S, Harapas CR, Hilton JB, Mlodzianoski MJ, Laohamonthonkul P, Louis C, Low RRJ, Moecking J, De Nardo D, Balka KR, Calleja DJ, Moghaddas F, Ni E, McLean CA, Samson AL, Tyebji S, Tonkin CJ, Bye CR, Turner BJ, Pepin G, Gantier MP, Rogers KL, McArthur K, Crouch PJ, Masters SL
Cell 2020 Oct 29;183(3):636-649.e18
Cell 2020 Oct 29;183(3):636-649.e18
VDAC2 enables BAX to mediate apoptosis and limit tumor development
Chin H, Li M, Tan I, Ninnis R, Reljic B, Scicluna K, Dagley L, Sandow J, Kelly G, Samson A, Chappaz S, Khaw S, Chang C, Morokoff A, Brinkmann K, Webb A, Hockings C, Hall C, Kueh A, Ryan M, Kluck R, Bouillet P, Herold M, Gray D, Huang D, van Delft M, Dewson G
Nature Communications 2018;9(1)
Nature Communications 2018;9(1)
Autophagy induced during apoptosis degrades mitochondria and inhibits type I interferon secretion
Lindqvist L, Frank D, McArthur K, Dite T, Lazarou M, Oakhill J, Kile B, Vaux D
Cell Death & Differentiation 2017;25(4):784-796
Cell Death & Differentiation 2017;25(4):784-796
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting TIMM44 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting TIMM44 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:394
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TIMM44 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TIMM44 antibody. Remaining relative intensity is presented
- Sample type
- Human
- Protocol
- Protocol
