PA5-69357

antibody from Invitrogen Antibodies

Targeting: NAAA ASAHL

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-69357

Provider

Invitrogen Antibodies

Product name

ASAHL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This target displays homology in the following species: Cow: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 93%; Rat: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

NAAA-regulated lipid signaling governs the transition from acute to chronic pain.

Fotio Y, Jung KM, Palese F, Obenaus A, Tagne AM, Lin L, Rashid TI, Pacheco R, Jullienne A, Ramirez J, Mor M, Spadoni G, Jang C, Hohmann AG, Piomelli D
Science advances 2021 Oct 22;7(43):eabi8834

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7. A critical period for pain chronification. ( A ) Mice received formalin (1%) or saline and were assigned to seven groups ( n = 10 mice each): five received one dose of ARN19702 (30 mg kg -1 , IP) or Veh on PFD1 or PFD2 to PFD5; two additional groups received one daily dose of ARN19702 or Veh on PFD6-7 or PFD8-9. Contralateral hyperalgesia was assessed 2 weeks later. ( B ) Effects of ARN19702 (blue) or Veh (red) on CPLS establishment. Open circles, no formalin. ( C ) Time course of Naaa transcription in ipsilateral (left) or contralateral (right) L4-L6 hemicord of mice treated with Veh (open circles) or formalin (red circles). ( D to G ) Localization of immunoreactive NAAA (irNAAA) in ipsilateral L4-L6 hemicord of (D) Veh- and (E) formalin-injected mice (PFD4). NAAA partially colocalized with NeuN; other markers are shown in fig. S7. Magnification: x10 (D and E) or x40 [(E, bottom), zoom 2x]. Scale bar, 20 mum. DAPI, 4',6-diamidino-2-phenylindole. (F) Quantification of irNAAA in ipsilateral dorsal horn (DH), ventral horn (VH), and white matter (WM) in L5 cord of Veh- or formalin-injected mice. (G) Quantification of NAAA-immunoreactive NeuN + cells in ipsilateral DH and VH in L5 cord of Veh- or formalin-injected mice. ( H ) PEA levels in ipsilateral L4-L6 hemicord fragments of Veh- (open circles) or formalin-treated mice (red circles) (PFD4). ( I ) Effects of intrathecal ARN19702 or ARN077 (30 ng, 5 mul) at PFD2 and PFD4 on hypersensitivity at PFD14. ( J ) Effects of in