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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-28283 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEF1E1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Raji, mouse brain. Predicted reactivity: Mouse (88%), Rat (87%), Pig (92%), Rhesus Monkey (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Disease-associated mutations in a bifunctional aminoacyl-tRNA synthetase gene elicit the integrated stress response.
Jin D, Wek SA, Kudlapur NT, Cantara WA, Bakhtina M, Wek RC, Musier-Forsyth K
The Journal of biological chemistry 2021 Oct;297(4):101203
The Journal of biological chemistry 2021 Oct;297(4):101203
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EEF1E1 in paraformaldehyde-fixed HeLa cells using an EEF1E1 polyclonal antibody (Product # PA5-28283) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 MSC association and protein integrity of EPRS-FLAG protein overexpressed in HEK293 T cells. A , doxycycline-inducible EPRS knockdown in HEK293T cells using shEPRS ( left ) or a nonspecific shRNA ( right ). B , immunoprecipitation experiments with FLAG antibody in HEK293T cells with endogenous EPRS knockdown and WT and mutant EPRS-FLAG overexpression. EPRS ( top ) and MSC scaffold proteins AIMP2 ( middle ) and AIMP3 ( bottom ) were visualized by immunoblotting using the indicated antibodies.
