MA5-24797

antibody from Invitrogen Antibodies

Targeting: PLIN2 ADFP, ADRP

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24797

Provider

Invitrogen Antibodies

Product name

ADFP Monoclonal Antibody (2C5A3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2C5A3

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2.

Eynaudi A, Díaz-Castro F, Bórquez JC, Bravo-Sagua R, Parra V, Troncoso R
Frontiers in nutrition 2021;8:775382

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Perilipin-2/ADFP in HepG2 cells using Perilipin-2/ADFP monoclonal antibody (Product # MA5-24797). Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The sample was then incubated with anti-ADFP/Perilipin-2 monoclonal antibody (Product # MA5-24797) and detected with anti-mouse Dylight 488 (1:500) and Phalloidin (1:200). Images were taken at 40x magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Oleic and palmitic acids induce different patterns of lipid droplet-mitochondria tether proteins in HepG2 cells. (A) Representative Western blots of the proteins associated with lipid droplet (LD)-mitochondria physical interaction: Perilipin 2 and 5 (PLIN2, PLIN5), and Mitofusin 2 (MFN2) in HepG2 cells treated with 200 muM of oleic acid (OA) or palmitic acid (PA) for 0-24 h. GAPDH was used as a loading control. (B-D) Densitometric quantification of the proteins indicated in A . Data are expressed as mean +- SEM of N = 7 for PLIN2 and N = 4 for PLIN5 and MFN2. * p < 0.05; ** p < 0.01 vs. BSA; # p < 0.05; and ## p < 0.01 vs. OA at the same time conditions.