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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
- Protein array [1]
- Other assay [1]
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- Product number
- V7686 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Adipophilin Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Adipophilin antibody is suitable for use in ELISA/Flow cytometry/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- ADFP/1365
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the Adipophilin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of a recombinant partial protein (immunogen, left) and human Jurkat cell lysate (right) with Adipophilin antibody (clone ADFP/1365). Predicted/observed molecular weight ~48 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of permeabilized human PMBC with Adipophilin antibody (clone ADFP/1365); Red=isotype control, Blue= Adipophilin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Adipophilin antibody (clone ADFP/1365). These results demonstrate the foremost specificity of the ADFP/1365 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Adipophilin antibody (clone ADFP/1365) as confirmation of integrity and purity.
