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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-25042 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADFP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Burkholderia pseudomallei interferes with host lipid metabolism via NR1D2-mediated PNPLA2/ATGL suppression to block autophagy-dependent inhibition of infection.
Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects.
Remodeling of host phosphatidylcholine by Chlamydia acyltransferase is regulated by acyl-CoA binding protein ACBD6 associated with lipid droplets.
Tang M, Hu Z, Rao C, Chen J, Yuan S, Zhang J, Mao C, Yan J, Xia Y, Zhang M, Yue J, Xiang Y, Xie J, Mao X, Li Q
Autophagy 2021 Aug;17(8):1918-1933
Autophagy 2021 Aug;17(8):1918-1933
Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects.
Bakke SS, Feng YZ, Nikolić N, Kase ET, Moro C, Stensrud C, Damlien L, Ludahl MO, Sandbu R, Solheim BM, Rustan AC, Hjelmesæth J, Thoresen GH, Aas V
PloS one 2015;10(3):e0119556
PloS one 2015;10(3):e0119556
Remodeling of host phosphatidylcholine by Chlamydia acyltransferase is regulated by acyl-CoA binding protein ACBD6 associated with lipid droplets.
Soupene E, Wang D, Kuypers FA
MicrobiologyOpen 2015 Apr;4(2):235-251
MicrobiologyOpen 2015 Apr;4(2):235-251
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ADFP polyclonal antibody (Product # PA5-25042) in Jurkat cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded breast carcinoma using an ADFP polyclonal antibody (Product # PA5-25042), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Jurkat cells using an ADFP polyclonal antibody (Product # PA5-25042) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 3 Higher lipolysis rate in myotubes from severely obese donors with type 2 diabetes. ( A ) Total lipolysis (with triacsin C) after 24 h incubation with [ 14 C]OA in non-diabetic (nD) and type 2 diabetic myotubes (T2D) n = 5-6. ( B ) Fasting plasma glucose levels correlated positively with total lipolysis rate, n = 11 (C) mRNA expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), perilipin 2 (PLIN2), perilipin 3 (PLIN3) and the fatty acid transporter CD36. Data are presented relative to mean values of non-diabetics, n = 6-10. ( D-E ) Protein expression of HSL, ATGL, comparative gene identification 58 (CGI-58), PLIN2 and PLIN3 and phosphorylation of HSL at serine 660 (HSL Ser660) after 24h incubation with 100 muM OA. (D) Two representative blots are shown. Data are presented relative to mean values of non-diabetics, n = 5.
