NBP1-47293
antibody from Novus Biologicals
Targeting: RMDN3
FAM82A2, FAM82C, FLJ10579, PTPIP51, RMD3
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-47293 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47293, RRID:AB_10010247
- Product name
- Rabbit Polyclonal PTPIP51 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PTPIP51 Antibody [NBP1-47293] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTPIP51 antibody NBP1-47293 used for IP at 3 ug/mg lysate. PTPIP51 was also immunoprecipitated by rabbit anti-PTPIP51 antibody NBP1-47294, which recognizes a downstream epitope. For blotting immunoprecipitated PTPIP51, NBP1-47294 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PTPIP51 Antibody [NBP1-47293] - Detection of human PTPIP51 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPIP51 antibody NBP1-47293 used for IP at 3 ug per reaction. PTPIP51 was also immunoprecipitated by rabbit anti-PTPIP51 antibody NBP1-47294. For blotting immunoprecipitated PTPIP51, NBP1-47293 was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PTPIP51 Antibody [NBP1-47293] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-PTPIP51 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PTPIP51 Antibody [NBP1-47293] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-PTPIP51 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB