Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF-481-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CCL25/TECK Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TECK is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting.
Chemokine 25-induced signaling suppresses colon cancer invasion and metastasis.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
Chen HJ, Sun J, Huang Z, Hou H Jr, Arcilla M, Rakhilin N, Joe DJ, Choi J, Gadamsetty P, Milsom J, Nandakumar G, Longman R, Zhou XK, Edwards R, Chen J, Chen KY, Bu P, Wang L, Xu Y, Munroe R, Abratte C, Miller AD, Gümüş ZH, Shuler M, Nishimura N, Edelmann W, Shen X, Lipkin SM
Nature biotechnology 2015 Jun;33(6):656-60
Nature biotechnology 2015 Jun;33(6):656-60
Chemokine 25-induced signaling suppresses colon cancer invasion and metastasis.
Chen HJ, Edwards R, Tucci S, Bu P, Milsom J, Lee S, Edelmann W, Gümüs ZH, Shen X, Lipkin S
The Journal of clinical investigation 2012 Sep;122(9):3184-96
The Journal of clinical investigation 2012 Sep;122(9):3184-96
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
Liu C, Ueno T, Kuse S, Saito F, Nitta T, Piali L, Nakano H, Kakiuchi T, Lipp M, Hollander GA, Takahama Y
Blood 2005 Jan 1;105(1):31-9
Blood 2005 Jan 1;105(1):31-9
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL25/TECK and Neutral-ization by Mouse CCL25/ TECK Antibody. Recombinant Mouse CCL25/TECK (Catalog # 481-TK) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL25/TECK (1.6 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL25/TECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-481-NA). 55 μg/mL of this antibody will neutralize rmTECK-induced chemotactic effect on BaF/3 hCCR9 cells by approximately 80-90%.