ABIN2509248

antibody from antibodies-online

Targeting: CCL25 Ckb15, SCYA25, TECK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2509248

Provider

antibodies-online

Product name

anti-Chemokine (C-C Motif) Ligand 25 (CCL25) antibody (Biotin)

Antibody type

Polyclonal

Antigen

Other

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK. Anti-Human TECK specific antibody was purified by affinity chromatography and then biotinylated.

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

50 μg

Storage

-20°C

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human Jurkat T cell leukemia cell lysate (lanes 3 and 6): 1-3. Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:400). 4-6. Anti-VPAC1 (extracellular) antibody, preincubated with the control peptide antigen. Expression of VPAC1 in rat amygdala Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:100). A. VPAC1 staining (red) is apparent in basolateral amygdala neurons (horizontal arrow). B. Cell nuclei in the same section are stained with DAPI (Blue). C. Merge of the two images. Expression of VPAC1 in human HT-29 cells Immunocytochemical staining of live intact human HT-29 colorectal adenocarcinoma cells. A. Extracellular staining of live cells with Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells. Indirect flow cytometry analysis of live intact Jurkat (human T cell leukemia cells) cell line: ___ Cells + goat-anti-rabbit-DyLight-488. ___ Cells + Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:20) + goat-anti-rabbit-DyLight-488.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Flow cytometry