Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN2509248 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Chemokine (C-C Motif) Ligand 25 (CCL25) antibody (Biotin)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK. Anti-Human TECK specific antibody was purified by affinity chromatography and then biotinylated.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 50 μg
- Storage
- -20°C
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human Jurkat T cell leukemia cell lysate (lanes 3 and 6): 1-3. Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:400). 4-6. Anti-VPAC1 (extracellular) antibody, preincubated with the control peptide antigen. Expression of VPAC1 in rat amygdala Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:100). A. VPAC1 staining (red) is apparent in basolateral amygdala neurons (horizontal arrow). B. Cell nuclei in the same section are stained with DAPI (Blue). C. Merge of the two images. Expression of VPAC1 in human HT-29 cells Immunocytochemical staining of live intact human HT-29 colorectal adenocarcinoma cells. A. Extracellular staining of live cells with Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells. Indirect flow cytometry analysis of live intact Jurkat (human T cell leukemia cells) cell line: ___ Cells + goat-anti-rabbit-DyLight-488. ___ Cells + Anti-VPAC1 (extracellular) antibody (ABIN2511208), (1:20) + goat-anti-rabbit-DyLight-488.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Flow cytometry