Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P134-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TECK Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSKRNVSLL ISANSGL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TECK (CCL25). Anti-Human TECK (CCL25)-specific antibody was purified by affinity chromatography employing an immobilized Human TECK (CCL25) matrix. Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TECK (CCL25) (500-P134Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL15). Western Blot: To detect Human TECK (CCL25) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TECK (CCL25) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Hepatic endothelial CCL25 mediates the recruitment of CCR9+ gut-homing lymphocytes to the liver in primary sclerosing cholangitis.
Eksteen B, Grant AJ, Miles A, Curbishley SM, Lalor PF, Hübscher SG, Briskin M, Salmon M, Adams DH
The Journal of experimental medicine 2004 Dec 6;200(11):1511-7
The Journal of experimental medicine 2004 Dec 6;200(11):1511-7
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TECK Polyclonal Antibody (Product # 500-P134-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TECK Polyclonal Antibody, Biotin (Product # 500-P134BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL15).
