Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- 500-P134-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TECK Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSKRNVSLL ISANSGL.
- Concentration
- 0.1-1.0 mg/mL
Submitted references Hepatic endothelial CCL25 mediates the recruitment of CCR9+ gut-homing lymphocytes to the liver in primary sclerosing cholangitis.
Eksteen B, Grant AJ, Miles A, Curbishley SM, Lalor PF, Hübscher SG, Briskin M, Salmon M, Adams DH
The Journal of experimental medicine 2004 Dec 6;200(11):1511-7
The Journal of experimental medicine 2004 Dec 6;200(11):1511-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human TECK (CCL25) by Western Blot analysis TECK Polyclonal Antibody (Product # 500-P134-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TECK (CCL25) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human TECK (CCL25) by Western Blot analysis TECK Polyclonal Antibody (Product # 500-P134-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TECK (CCL25) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TECK Polyclonal Antibody (Product # 500-P134-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TECK Polyclonal Antibody, Biotin (Product # 500-P134BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL15).