701117

antibody from Invitrogen Antibodies

Targeting: CXCL9 CMK, crg-10, Humig, MIG, SCYB9

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

701117

Provider

Invitrogen Antibodies

Product name

CXCL9 Recombinant Rabbit Monoclonal Antibody (11H1L14)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

11H1L14

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice.

Cai W, Shi L, Zhao J, Xu F, Dufort C, Ye Q, Yang T, Dai X, Lyu J, Jin C, Pu H, Yu F, Hassan S, Sun Z, Zhang W, Hitchens TK, Shi Y, Thomson AW, Leak RK, Hu X, Chen J
The Journal of clinical investigation 2022 Aug 1;132(15)

---

Senescent hepatocytes enhance natural killer cell activity via the CXCL-10/CXCR3 axis.

Zang J, Ye J, Zhang C, Sha M, Gao J
Experimental and therapeutic medicine 2019 Nov;18(5):3845-3852

---

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8(+) T cell tumor-infiltration impairing anti-PD1 therapy.

Pascual-García M, Bonfill-Teixidor E, Planas-Rigol E, Rubio-Perez C, Iurlaro R, Arias A, Cuartas I, Sala-Hojman A, Escudero L, Martínez-Ricarte F, Huber-Ruano I, Nuciforo P, Pedrosa L, Marques C, Braña I, Garralda E, Vieito M, Squatrito M, Pineda E, Graus F, Espejo C, Sahuquillo J, Tabernero J, Seoane J
Nature communications 2019 Jun 11;10(1):2416

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CXCL9/MIG was performed using 70% confluent log phase THP-1 cells treated with 100 ng of IFN-gamma for 15 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CXCL9/MIG (11H1L14) Recombinant Rabbit Monoclonal Antibody (Product # 701117) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CXCL9 / MIG was done on THP-1 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with CXCL9 / MIG Rabbit Monoclonal Antibody (701117, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Expression of CXCL-9, -10 and -11 in senescent hepatocytes. (A) Expression of mRNA in senescent AML12 cells was measured by reverse transcription-quantitative PCR in comparison with controls. (B) Relative protein levels in senescent AML12 cells were determined by western blotting. n=3. Data were analyzed by Student's t-test; ***P