Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-492-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL9/MIG Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CXCL9/MIG in ELISAs. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) CXCL9/MIG is observed and less than 0.005% cross-reactivity with recombinant mouse MIP-2, recombinant rat CINC-1, rhGRO alpha , rhGRO beta , and rhGRO gamma is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P18340
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis.
Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production.
Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells.
Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone.
A Novel PD-L1-targeting Antagonistic DNA Aptamer With Antitumor Effects.
CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.
Regulatory T cells prevent inducible BALT formation by dampening neutrophilic inflammation.
Tumor cell-activated CARD9 signaling contributes to metastasis-associated macrophage polarization.
Trafficking of high avidity HER-2/neu-specific T cells into HER-2/neu-expressing tumors after depletion of effector/memory-like regulatory T cells.
A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Monokine induced by interferon-gamma (MIG/CXCL9) is derived from both donor and recipient sources during rejection of class II major histocompatibility complex disparate skin allografts.
L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells.
Viral infection of the lungs through the eye.
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis.
Koyanagi N, Imai T, Shindo K, Sato A, Fujii W, Ichinohe T, Takemura N, Kakuta S, Uematsu S, Kiyono H, Maruzuru Y, Arii J, Kato A, Kawaguchi Y
The Journal of clinical investigation 2017 Oct 2;127(10):3784-3795
The Journal of clinical investigation 2017 Oct 2;127(10):3784-3795
Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production.
Shinde P, Liu W, Ménoret A, Luster AD, Vella AT
Journal of leukocyte biology 2017 Jul;102(1):57-69
Journal of leukocyte biology 2017 Jul;102(1):57-69
Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells.
Arai Y, Takahashi D, Asano K, Tanaka M, Oda M, Ko SBH, Ko MSH, Mandai S, Nomura N, Rai T, Uchida S, Sohara E
Scientific reports 2017 Apr 20;7:46580
Scientific reports 2017 Apr 20;7:46580
Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone.
Huang B, Wang W, Li Q, Wang Z, Yan B, Zhang Z, Wang L, Huang M, Jia C, Lu J, Liu S, Chen H, Li M, Cai D, Jiang Y, Jin D, Bai X
Nature communications 2016 Dec 14;7:13885
Nature communications 2016 Dec 14;7:13885
A Novel PD-L1-targeting Antagonistic DNA Aptamer With Antitumor Effects.
Lai WY, Huang BT, Wang JW, Lin PY, Yang PC
Molecular therapy. Nucleic acids 2016 Dec 13;5(12):e397
Molecular therapy. Nucleic acids 2016 Dec 13;5(12):e397
CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.
Dai Z, Xing L, Cerise J, Wang EH, Jabbari A, de Jong A, Petukhova L, Christiano AM, Clynes R
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1089-99
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1089-99
Regulatory T cells prevent inducible BALT formation by dampening neutrophilic inflammation.
Foo SY, Zhang V, Lalwani A, Lynch JP, Zhuang A, Lam CE, Foster PS, King C, Steptoe RJ, Mazzone SB, Sly PD, Phipps S
Journal of immunology (Baltimore, Md. : 1950) 2015 May 1;194(9):4567-76
Journal of immunology (Baltimore, Md. : 1950) 2015 May 1;194(9):4567-76
Tumor cell-activated CARD9 signaling contributes to metastasis-associated macrophage polarization.
Yang M, Shao JH, Miao YJ, Cui W, Qi YF, Han JH, Lin X, Du J
Cell death and differentiation 2014 Aug;21(8):1290-302
Cell death and differentiation 2014 Aug;21(8):1290-302
Trafficking of high avidity HER-2/neu-specific T cells into HER-2/neu-expressing tumors after depletion of effector/memory-like regulatory T cells.
Weiss VL, Lee TH, Song H, Kouo TS, Black CM, Sgouros G, Jaffee EM, Armstrong TD
PloS one 2012;7(2):e31962
PloS one 2012;7(2):e31962
A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models.
Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):4935-46
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):4935-46
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Monokine induced by interferon-gamma (MIG/CXCL9) is derived from both donor and recipient sources during rejection of class II major histocompatibility complex disparate skin allografts.
Auerbach MB, Shimoda N, Amano H, Rosenblum JM, Kish DD, Farber JM, Fairchild RL
The American journal of pathology 2009 Jun;174(6):2172-81
The American journal of pathology 2009 Jun;174(6):2172-81
L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells.
Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martín-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F
Nature immunology 2007 Jul;8(7):743-52
Nature immunology 2007 Jul;8(7):743-52
Viral infection of the lungs through the eye.
Bitko V, Musiyenko A, Barik S
Journal of virology 2007 Jan;81(2):783-90
Journal of virology 2007 Jan;81(2):783-90
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Fujita H, Asahina A, Komine M, Tamaki K
Cellular immunology 2007 Feb;245(2):70-9
Cellular immunology 2007 Feb;245(2):70-9
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
Janatpour MJ, Hudak S, Sathe M, Sedgwick JD, McEvoy LM
The Journal of experimental medicine 2001 Nov 5;194(9):1375-84
The Journal of experimental medicine 2001 Nov 5;194(9):1375-84
Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
Janatpour MJ, Hudak S, Sathe M, Sedgwick JD, McEvoy LM
The Journal of experimental medicine 2001 Nov 5;194(9):1375-84
The Journal of experimental medicine 2001 Nov 5;194(9):1375-84
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CXCL9/MIG by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL9/MIG at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. Recombinant Mouse CXCL9/MIG (Catalog # 492-MM) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND50 is typically 6-20 µg/mL.