MA5-27620

antibody from Invitrogen Antibodies

Targeting: TRPM7 CHAK1, LTRPC7, TRP-PLIK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27620

Provider

Invitrogen Antibodies

Product name

TRPM7 Monoclonal Antibody (S74)

Antibody type

Monoclonal

Antigen

Other

Description

1 µg/mL of MA5-27620 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 220kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S74

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics.

Kollewe A, Chubanov V, Tseung FT, Correia L, Schmidt E, Rössig A, Zierler S, Haupt A, Müller CS, Bildl W, Schulte U, Nicke A, Fakler B, Gudermann T
eLife 2021 Nov 12;10

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Epidermal growth factor signaling through transient receptor potential melastatin 7 cation channel regulates vascular smooth muscle cell function.

Zou ZG, Rios FJ, Neves KB, Alves-Lopes R, Ling J, Baillie GS, Gao X, Fuller W, Camargo LL, Gudermann T, Chubanov V, Montezano AC, Touyz RM
Clinical science (London, England : 1979) 2020 Aug 14;134(15):2019-2035

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TRPM7 in human cell lysates with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TRPM7 in paraffin-embedded human hippocampus. Sample was fixed with Bouin's Fixative Solution, incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TRPM7 in paraffin-embedded mouse hippocampus. Samples were incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (1 hour at RT) and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) at a dilution of 250-500 µl.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TRPM7 in paraffin-embedded human hippocampus. Sample was fixed with Bouin's Fixative Solution, incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 EGF enhances direct interaction between EGFR and TRPM7 in a c-Src-dependent manner ( A ) EGFR was detected when TRPM7 was immunoprecipitated in rVSMCs using anti-TRPM7 antibody (left panel) and TRPM7 was detected when EGFR was immunoprecipitated using anti-EGFR antibody (right panel) ( n =3-4). ( B ) Representative confocal microscopy images of rVSMCs co-immunostained for TRPM7 (Alexa 488, green) and EGFR (Alexa 555, red). Nuclei were stained with DAPI ( n =3). ( C , D ) PLA was used to visualize and quantify TRPM7-EGFR interaction in rVSMCs stimulated with vehicle (veh), and EGF (50 ng/ml) in the presence and absence of gefitinib (1 uM) and PP2 (10 uM). WGA (Alexa 488, green) was used to stain cell membranes. Red fluorescence is identified as PLA positive signals. Orange fluorescence in the merged figure identifies co-localization. PLA signals were quantified using the Analyse Particles plugin of ImageJ software. For each condition, quantifications were performed from at least 100 cells and expressed as the mean number of signals per cell (percentage of control). Scale bar =10 um. Arrows indicate co-localization of EGFR and TRPM7. * P