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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ACC-047-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPM7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat GH3pituitary tumor cell line (lanes 1 and 2) and mouse brain (lanes 3 and 4) lysates: - 1,3. Anti-TRPM7 Antibody (#ACC-047), (1:200).2,4. Anti-TRPM7 Antibody , preincubated with TRPM7 Blocking Peptide (#BLP-CC047).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPM7 in GH3 cell lines - Immunocytochemical staining of GH3 cells with Anti-TRPM7 Antibody (#ACC-047), (1:100), followed by goat Anti-rabbit-AlexaFluor-550 secondary Antibody (A). B. Nuclear staining of GH3 cells with the cell-permeable dye Hoechst 33342. C. Live intact GH3 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPM7 in rat pituitary gland - Immunohistochemical staining of rat hypophysis paraffin embedded transversal sections using Anti-TRPM7 Antibody (#ACC-047), (1:50). TRPM7 is stained in both the posterior part or neurohypophysis (N) and the anterior part or adenohypophysis (A). Note that stain is highly specific for andocrine cells in the adenohypophysis. Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the Counterstain.
