MA5-47006

antibody from Invitrogen Antibodies

Targeting: GSPT1 eRF3a, ETF3A, GST1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-47006

Provider

Invitrogen Antibodies

Product name

GSPT1 Recombinant Rabbit Monoclonal Antibody (HL1346)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Store as concentrated solution. Centrifuge briefly prior to opening vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

HL1346

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GSPT1 in Various whole cell extracts (30 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with GSPT1 Monoclonal antibody (Product # MA5-47006) using a dilution of 1:20,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GSPT1 in Various whole cell extracts (30 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with GSPT1 Monoclonal antibody (Product # MA5-47006) using a dilution of 1:3,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GSPT1 in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 monoclonal antibody (Product # MA5-47006) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GSPT1 in paraffin-embedded human breast carcinoma. Samples were incubated in GSPT1 Monoclonal antibody (Product # MA5-47006). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GSPT1 in paraffin-embedded rat colon. Samples were incubated in GSPT1 Monoclonal antibody (Product # MA5-47006). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GSPT1 in paraffin-embedded mouse esophagus. Samples were incubated in GSPT1 Monoclonal antibody (Product # MA5-47006). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GSPT1 in paraffin-embedded rat tissues. Samples were incubated in GSPT1 Monoclonal antibody (Product # MA5-47006). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.