Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-28256 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSPT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (91%), Rat (93%), Chicken (81%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSPT1 using A) 30 µg A549 whole cell extract (B) 30 µg HeLa whole cell extract (C) 30 µg HepG2 whole cell extract and D) 30 µg HCT116 whole cell extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GSPT1 polyclonal antibody (Product # PA5-28256) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSPT1 in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with GSPT1 Polyclonal antibody (Product # PA5-28256) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSPT1 Polyclonal Antibody detects GSPT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 Polyclonal Antibody (Product # PA5-28256) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSPT1 Polyclonal Antibody detects GSPT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 Polyclonal Antibody (Product # PA5-28256) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GSPT1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 Polyclonal Antibody (Product # PA5-28256) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSPT1 Polyclonal Antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GSPT1 stained by GSPT1 Polyclonal Antibody (Product # PA5-28256) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using GSPT1 (Product # PA5-28256) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.