PA5-97034

antibody from Invitrogen Antibodies

Targeting: YTHDF3 FLJ31657

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97034

Provider

Invitrogen Antibodies

Product name

YTHDF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSATSVDQRP KGQGNKVSVQ NGSIHQKDAV NDDDFEPYLS SQTNQSNSYP PMSDPYMPSY YAPSIGFPYS LGEAAWSTAG DQPMPYLTTY GQMSNGEHHY IPDGVFSQPG ALGNTPPFLG QHGFNFFPGN ADFSTWGTSG SQGQSTQSSA YSSSYGYPPS SLGRAITDGQ AGFGNDTLSK VPGISSIEQG MTGLKIGGDL TAAVTKTVGT ALSSSGMTSI ATNSVPPVSS AAPKPTSWAA IARKPAKPQP KLKPKGNVGI GGSAVPPPPI KHNMN; Positive Samples: HT-1080, HepG2

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

5.83 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of YTHDF3 in extracts of Mouse liver. Samples were incubated with YTHDF3 Polyclonal antibody (Product # PA5-97034) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of YTHDF3 in extracts of HepG2 cells. Samples were incubated with YTHDF3 Polyclonal antibody (Product # PA5-97034) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of YTHDF3 in HeLa cells. Samples were incubated with YTHDF3 Polyclonal antibody (Product # PA5-97034) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of YTHDF3 in NIH/3T3 cells. Samples were incubated with YTHDF3 Polyclonal antibody (Product # PA5-97034) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of YTHDF3 in 300 μg extracts of HT-1080 cells. Samples were precipitated with 3 μg YTHDF3 Polyclonal antibody (Product # PA5-97034). Western blot was performed from the immunoprecipitate using YTHDF3 Polyclonal antibody (Product # PA5-97034) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of YTHDF3 in paraffin-embedded mouse brain. Samples were incubated with YTHDF3 Polyclonal antibody (Product # PA5-97034) using a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of YTHDF3 in paraffin-embedded rat brain. Samples were incubated with YTHDF3 Polyclonal antibody (Product # PA5-97034) using a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of YTHDF3 in paraffin-embedded mouse brain. Samples were incubated with YTHDF3 Polyclonal antibody (Product # PA5-97034) using a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.