AF5094

antibody from Novus Biologicals

Targeting: RASA1 CM-AVM, GAP, p120, p120GAP, p120RASGAP, RASA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5094

Provider

Novus Biologicals

Product name

Goat Polyclonal Ras-GAP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human, mouse, and rat Ras-GAP in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Small RNA sequencing reveals microRNAs that modulate angiotensin II effects in vascular smooth muscle cells.

Jin W, Reddy MA, Chen Z, Putta S, Lanting L, Kato M, Park JT, Chandra M, Wang C, Tangirala RK, Natarajan R
The Journal of biological chemistry 2012 May 4;287(19):15672-83

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat Ras-GAP by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Ras-GAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5094) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ras-GAP at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.