Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX22922 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22922, RRID:AB_371449
- Product name
- RASA1 antibody [B4F8]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Simian
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of GAP in 25 ug of HeLa, A431 and mouse brain lysates. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4?C overnight. The membrane was probed with GAP antibody [B4F8] at a dilution of 1:200 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GAP (green) in HeLa cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with GAP antibody [B4F8] in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4?C in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.