Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-88982 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88982, RRID:AB_11003612
- Product name
- Rabbit Polyclonal RHOT2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human RHOT2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.
Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT
The Journal of biological chemistry 2014 May 23;289(21):14569-82
The Journal of biological chemistry 2014 May 23;289(21):14569-82
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: RHOT2 Antibody [NBP1-88982] - Simple Western lane view shows a specific band for RHOT2 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: RHOT2 Antibody [NBP1-88982] - Electropherogram image(s) of corresponding Simple Western lane view. RHOT2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RHOT2 Antibody [NBP1-88982] - Analysis in human cell line HEK 293.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human small intestine shows cytoplasmic positivity in glandular cells.