Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- Ab090177 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- ATP6V1D Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human ATP6V1D Antibody, Polyclonal, could be used for WB, IHC and so on.Application:WB: 1/500-1/4000IHC: 1/50-1/100Protein FunctionRelevance': ""ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 169-238
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,9 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 113.8 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 17.7 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ATP6V1D Antibody (Ab090177) - IHC Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer using ATP6V1D Antibody (Ab090177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.