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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
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- Product number
- PA5-47505 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX1, rhSOX2 and rhSOX15 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 0.2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SOX21 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-human SOX21 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SOX21 in immersion fixed NTera‚2 human testicular embryonic carcinoma cell line. Samples were incubated in SOX21 polyclonal antibody (Product # PA5-47505) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SOX21 in immersion fixed NTera‚2 human testicular embryonic carcinoma cell line. Samples were incubated in SOX21 polyclonal antibody (Product # PA5-47505) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SOX21 in immersion fixed NTera‚2 human testicular embryonic carcinoma cell line. Samples were incubated in SOX21 polyclonal antibody (Product # PA5-47505) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
