Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- MA5-26509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIK3C2A Monoclonal Antibody (OTI3D9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3D9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3C2A in HT29 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with PIK3C2A (Product # MA5-26509) monoclonal antibody at a dilution of 1:500.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3C2A in HeLa wild-type and knockout cells using 10 µg per lane. Samples were separated by SDS-PAGE and probed with PIK3C2A (Product # MA5-26509) monoclonal antibody and HSP90AB1 antibody with a dilution of 1:500 as a loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3C2A in E.coli lysate using. Samples were separated by SDS-PAGE and probed with PIK3C2A (Product # MA5-26509) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3C2A in HeLa wild-type and knockout cells using 10 µg per lane. Samples were separated by SDS-PAGE and probed with PIK3C2A (Product # MA5-26509) monoclonal antibody and HSP90AB1 antibody with a dilution of 1:500 as a loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a PIK3C2A monoclonal antibody (Product # MA5-26509).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a PIK3C2A monoclonal antibody (Product # MA5-26509).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a PIK3C2A monoclonal antibody (Product # MA5-26509).