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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [2]
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- Product number
- 22028-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIK3C2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAQISSNSG FKECPSSHPE PTRAKDVDKE EALQMEAEAL AKLQKDRQVT DNQRGFELSS STRKKAQVYN KQDYDLMVFP ESDSQKRALD IDVEKLTQAE LEKLLLDDSF ETKKTPVLPV TPILSPSFSA QLYFRPTIQR GQWPPGLPGP STYALPSIYP STYSKQAAFQ NGFNPRMPTF PSTEPIYLSL PGQSPYFSYP LTPATPFHPQ GSLPIYRPVV STDMAKLFDK IASTSEFLKN GKARTDLEIT DSKVSNLQVS PKSEDISKFD WLDLDPLSKP KVDNVEVLDH EEEKNVSSLL AKDPWDAVLL EERSTANCHL ERKVNGKSLS VATVTRSQS (1-338 aa encoded by BC113658 )
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.39 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 22028-1-AP (PIK3C2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 22028-1-AP (PIK3C2A antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 22028-1-AP (PIK3C2A antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 22028-1-AP (PIK3C2A antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 22028-1-AP (PIK3C2A antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 22028-1-AP (PIK3C2A antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PIK3C2A (IP:22028-1-AP, 4ug; Detection:22028-1-AP 1:1000) with PC-3 cells lysate 3200ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PIK3C2A (IP:22028-1-AP, 4 ug; Detection:22028-1-AP 1:1000) with PC-3 cells lysate 1760 ug.
