17150-1-AP

antibody from Invitrogen Antibodies

Targeting: YARS2 CGI-04, FLJ13995, mt-TyrRS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17150-1-AP

Provider

Invitrogen Antibodies

Product name

YARS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EREALETER VRANARALRL GLEALAANHQ QLFTDGRSWG SFTVLDNSAW YQKQHLVDFL AAVGGHFRMG TLLSRQSVQL RLKSPEGMSL AEFFYQVLQA YDFYYLFQRY GCRVQLGGSD QLGNIMSGYE FINKLTGEDV FGITVPLITS TTGAKLGKSA GNAVWLNRDK TSPFELYQFF VRQPDDSVER YLKLFTFLPL PEIDHIMQLH VKEPERRGPQ KRLAAEVTKL VHGREGLDSA KRCTQALYHS SIDALEVMSD QELKELFKEA PFSEFFLDPG TSVLDTCRKA NAIPDGPRGY RMITEGGVSI NHQQVTNPES VLIVGQHILK NGLSLLKIGK RNFYIIKWLQ L (128-477 aa encoded by BC015625)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 17150-1-AP (YARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 17150-1-AP (YARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-YARS2 (IP:17150-1-AP, 4ug; Detection:17150-1-AP 1:1000) with K-562 cells lysate 4400 ug.