11739-1-AP

antibody from Invitrogen Antibodies

Targeting: ASGR1 CLEC4H1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11739-1-AP

Provider

Invitrogen Antibodies

Product name

ASGR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MTKEYQDLQ HLDNEESDHH QLRKGPPPPQ PLLQRLCSGP RLLLLSLGLS LLLLVVVCVI GSQNSQLQEE LRGLRETFSN FTASTEAQVK GLSTQGGNVG RKMKSLESQL EKQQKDLSED HSSLLLHVKQ FVSDLRSLSC QMAALQGNGS ERTCCPVNWV EHERSCYWFS RSGKAWADAD NYCRLEDAHL VVVTSWEEQK FVQHHIGPVN TWMGLHDQNG PWKWVDGTDY ETGFKNWRPE QPDDWYGHGL GGGEDCAHFT DDGRWNDDVC QRPYRWVCET ELDKASQEPP LL (1-291 aa encoded by BC032130)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP ( ASGR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 11739-1-AP (ASGR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 11739-1-AP (ASGR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11739-1-AP ( ASGR1 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11739-1-AP ( ASGR1 Antibody) at dilution of 1:200 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug ASGR1 antibody (11739-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 0.3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ASGR1 (IP:11739-1-AP, 4ug; Detection:11739-1-AP 1:500) with mouse liver tissue lysate 4000 ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ASGR1 (IP:11739-1-AP, 4 ug; Detection:11739-1-AP 1:2000) with mouse liver tissue lysate 4000 ug.