NBP1-85581

antibody from Novus Biologicals

Targeting: ASGR1 CLEC4H1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85581

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85581, RRID:AB_11021923

Product name

Rabbit Polyclonal ASGPR1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human ASGPR1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Expression of asialoglycoprotein receptor 1 in human hepatocellular carcinoma.

Shi B, Abrams M, Sepp-Lorenzino L
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Dec;61(12):901-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ASGPR1 Antibody [NBP1-85581] - Analysis in human cell line HepG2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ASGPR1 Antibody [NBP1-85581] - Analysis in control (vector only transfected HEK293T lysate) and ASGR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human liver shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human lymph node.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human tonsil shows no positivity in germinal center cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining in human liver and endometrium tissues using NBP1-85581 antibody. Corresponding ASGR1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human endometrium shows no positivity in glandular cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human endometrium, kidney, liver and tonsil using Anti-ASGR1 antibody NBP1-85581 (A) shows similar protein distribution across tissues to independent antibody NBP1-85580 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human kidney shows no positivity in cells in tubules as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.