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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- UM500021 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500021, RRID:AB_2629036
- Product name
- XPF mouse monoclonal antibody,clone UMAB21
- Antibody type
- Monoclonal
- Description
- XPF mouse monoclonal antibody,clone UMAB21
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ERCC4
- Isotype
- IgG
- Antibody clone number
- UMAB21
- Vial size
- 100 µl
- Concentration
- 0.38mg/ml
Submitted references TGF beta promotes repair of bulky DNA damage through increased ERCC1/XPF and ERCC1/XPA interaction.
Zheng H, Jarvis IWH, Bottai M, Dreij K, Stenius U
Carcinogenesis 2019 Jun 10;40(4):580-591
Carcinogenesis 2019 Jun 10;40(4):580-591
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of crude cell extracts (35ug) from 9 different cell lines by using anti-XPF (UMAB21) monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-XPF monoclonal antibody (clone UMAB21, 1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-XPF (UMAB21) mouse monoclonal antibody (UM500021) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF(RC223300).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-XPF (UMAB21) mouse monoclonal antibody. (UM500021, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XPF mouse monoclonal antibody (clone UMAB21). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XPF (UMAB21) very specifically recognizes XPF antigen on OriGene protein microarray chip.
- Validation comment
- 10K-CHIP
