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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- A301-103A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XRN2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse XRN2 by western blot. Samples: Whole cell lysate (15 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody (Product # A301-103A; lot A301-103A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human XRN2 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody (Product # A301-103A; lot A301-103A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse XRN2 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-XRN2 antibody A301-103A (lot A301-103A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human XRN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-XRN2 antibody A301-103A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. XRN2 was also immunoprecipitated by rabbit anti-XRN2 antibody BL2040, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse XRN2 by immunohistochemistry. Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (Product # A301-103A; lot 2) used at a dilution of 1:1000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human XRN2 by immunohistochemistry. Sample: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (Product # A301-103A Lot 2) used at a dilution of 1:1000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse XRN2 by immunohistochemistry. Sample: FFPE sections of human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- XRN2 (Cat. No. A301-103A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human XRN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN2 antibody (Products # A301-103A; lot A301-103A-2) used for IP at 3 µg per reaction. XRN2 was also immunoprecipitated by rabbit anti-XRN2 antibody BL2040. For blotting immunoprecipitated XRN2 (Product # A301-103A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
