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LearnPA5-100854
antibody from Invitrogen Antibodies
Targeting: PAK1IP1
bA421M1.5, FLJ20624, hPIP1, MAK11, PIP1, WDR84
Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-100854 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAK1IP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total PAK1IP1.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PAK1IP1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PAK1IP1 polyclonal antibody (Product # PA5-100854) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
