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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-35865 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Centrin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT-116, Neuro2A, CD830, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (83%), Xenopus laevis (89%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Centrin-3 using Various whole cell extracts (30 µg). Samples were loaded onto a 15% SDS-PAGE gel and probed with a Centrin-3 polyclonal antibody (Product # PA5-35865) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody Centrin 3 Polyclonal Antibody (Product # PA5-35865) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts of mouse and rat (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody Centrin 3 Polyclonal Antibody (Product # PA5-35865) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Centrin3 antibody detects Centrin3 protein at centrosome by immunofluorescent analysis. Sample: A549 cells were fixed in 100% MeOH for 5 min. Green: Centrin3 protein stained by Centrin3 antibody (Product # PA5-35865) diluted at 1:1,000. Red: alpha Tubulin Polyclonal Antibody [B-5-1-2] (Product # MA1-25054) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Centrin3 antibody detects Centrin3 protein at cytosol on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Centrin3 antibody (Product # PA5-35865) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
