A304-176A

antibody from Invitrogen Antibodies

Targeting: SEPTIN11 FLJ10849, SEPT11

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A304-176A

Provider

Invitrogen Antibodies

Product name

Septin 11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Septin 11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Septin 11 antibody A304-176A (lot A304-176A-1) used for IP at 6 µg/mg lysate. Septin 11 was also immunoprecipitated by rabbit anti-Septin 11 antibody A304-177A. For blotting immunoprecipitated Septin 11, A304-176A was used at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Septin 11 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Septin 11 antibody A304-176A (lot A304-176A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.