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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- PA5-29533 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Septin-11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2, mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (94%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.37 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEPT11 using 30 µg of A) 293T (B) HeLa and C) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SEPT11 polyclonal antibody (Product # PA5-29533) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Septin-11 Polyclonal Antibody (Product # PA5-29533). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. Septin-11 Polyclonal Antibody (Product # PA5-29533) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Septin-11 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Septin-11 Polyclonal Antibody (Product # PA5-29533) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Septin-11 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Septin-11 Polyclonal Antibody (Product # PA5-29533) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEPT11 antibody detects SEPT11 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. SEPT11 antibody (Product # PA5-29533) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
