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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-45721 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPS28 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: RTGSQGQCTQ VRVEFMDDTS RSIIRNVKGP VREGDVLTLL ESEREARRLR Sequence homology: Cow: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 93%; Zebrafish: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Proteomic and mechanistic dissection of the poxvirus-customized ribosome.
DiGiuseppe S, Rollins MG, Astar H, Khalatyan N, Savas JN, Walsh D
Journal of cell science 2020 Jul 9;134(5)
Journal of cell science 2020 Jul 9;134(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3). The blot was probed with Anti-RPS28 Polyclonal Antibody (Product # PA5-45721, 1 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 7 kDa band corresponding to RPS28 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MDA-MB435 cells using an anti-RPS28 polyclonal antibody (Product # PA5-45721).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RPS28 was achieved by transfecting HeLa cells with RPS28 specific siRNAs (Silencer® select Product # s226977). Western blot analysis (Fig. a) was performed using whole cell extracts from the RPS28 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RPS28 Polyclonal Antibody (Product # PA5-45721, 1µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPS28.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human adult liver tissue using an anti-RPS28 polyclonal antibody (Product # PA5-45721).
