MA5-14820

antibody from Invitrogen Antibodies

Targeting: DNAJC3 ERdj6, HP58, P58, p58(IPK), P58IPK, PRKRI

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14820

Provider

Invitrogen Antibodies

Product name

DNAJC3 Monoclonal Antibody (J.98.6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

J.98.6

Vial size

100 µL

Concentration

154 µg/mL

Storage

-20°C

References

Submitted references

Inhibition of eIF2α Dephosphorylation Protects Hepatocytes from Apoptosis by Alleviating ER Stress in Acute Liver Injury.

Tang YJ, Chen H, Yi Y, Chen GM, Yang FW, Li Y, Tian RD, Huang WG, Cheng QJ, He YH
BioMed research international 2020;2020:2626090

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Inhibiting alpha subunit of eukaryotic initiation factor 2 dephosphorylation protects injured hepatocytes and reduces hepatocyte proliferation in acute liver injury.

Chen G, Yang X, He Y, Tang Y, Tian R, Huang W, Chen H, Yang F, Li Y, Lin S
Croatian medical journal 2019 Dec 31;60(6):532-544

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 The inhibition of alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) dephosphorylation mitigates carbon tetrachloride (CCl 4 )-induced hepatocyte proliferation in mice. Male BALB/c mice were pretreated with vehicle (dimethyl sulfoxide + phosphate buffer solution), salubrinal, integrated stress response inhibitor (ISRIB) for 2 h, or recombinant AAV8 expressing DnaJC3 for 4 weeks, then injected with olive oil or CCl 4 . Relative levels of intrahepatic cyclin D1 and proliferating cell nuclear antigen (PCNA) expression at 24 h after CCl 4 injection were determined by Western blot. PCNA expression was assessed by immunohistochemistry. ( A ) Salubrinal, ISRIB, or overexpressed DnaJC3 altered eIF2alpha phosphorylation, DnaJC3, and PCNA expression (n = 6; total mice = 30). ( B ) Salubrinal, ISRIB, or ( C ) overexpressed DnaJC3 altered the levels of CCl 4 -induced eIF2alpha phosphorylation, cyclin D1, and PCNA expression (n = 8, total mice = 40, day 1). ( D ) Immunohistochemistry analysis of hepatic PCNA expression (magnification x100).