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Western blot
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- Antibody Data
- Antigen structure
- References [2]
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- Other assay [3]
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- Product number
- MA5-18052 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOX2 Monoclonal Antibody (54.1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody is specific for NOX2/gp91phox (aa 382-389, PKIAVDGP). The immunogen was prepared using partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100). Western blot results show a band at 65 kDa, which matches the predicted molecular weight. For IHC applications, perform heat-mediated antigen retrieval with citrate buffer, pH 6, prior to staining.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 54.1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.
Alisol B 23-Acetate Ameliorates Lipopolysaccharide-Induced Cardiac Dysfunction by Suppressing Toll-Like Receptor 4 (TLR4)/NADPH Oxidase 2 (NOX2) Signaling Pathway.
Wanschel ACBA, Guizoni DM, Lorza-Gil E, Salerno AG, Paiva AA, Dorighello GG, Davel AP, Balkan W, Hare JM, Oliveira HCF
Biomolecules 2021 Jan 7;11(1)
Biomolecules 2021 Jan 7;11(1)
Alisol B 23-Acetate Ameliorates Lipopolysaccharide-Induced Cardiac Dysfunction by Suppressing Toll-Like Receptor 4 (TLR4)/NADPH Oxidase 2 (NOX2) Signaling Pathway.
Wang B, Chen L, Dai L, Fang W, Wang H
Medical science monitor : international medical journal of experimental and clinical research 2019 Nov 10;25:8472-8481
Medical science monitor : international medical journal of experimental and clinical research 2019 Nov 10;25:8472-8481
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 CETP inhibition decreases NADPH oxidase activation. Representative microscopic images of HAEC stained with ( A ) p47 phox ( B ) NOX2 (gp91 phox ) and DAPI. p47 phox and NOX2 (green) are localized to the plasma membrane, stained with wheat germ agglutinin (WGA-red). After CETP inhibition, p47 phox and NOX2 co-localize with cytoplasmic aggregates of endogenous actin, detectable with phalloidin labeling. Upper panel 10X magnification and bottom 20X. Scale bar, 10 mum. ( C ) Western blotting of p47 phox and NOX2 (gp91 phox ) in the plasma membrane fraction of HAEC normalized by the L-type calcium channel protein ( n = 3, * p < 0.05). HAECs, human aortic endothelial cells. CETP, cholesteryl ester transfer protein. NS, non-silenced. siCETP, silenced CETP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 ( A, B ) AB23A attenuates the lipopolysaccharide (LPS)-induced alteration in TLR4/NOX2 signaling. The western blotting assay showed the pre-treatment of AB23A to reduce the increasing expressions of TLR4, NOX2, p-P38, and p-ERK protein in LPS-induced septic mice (n=3).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 ( A, B ) AB23A regulates TLR4, NOX2, p-P38, and p-ERK protein expression in the H9C2 cell line with LPS stimulation. The western blotting assay showed the pre-treatment of AB23A to reduce the increasing expressions of TLR4, NOX2, p-P38, and p-ERK protein in H9C2 cells (n=3).
