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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-79118 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human Raji whole cell, human Jurkat whole cell, human MCF-7 whole cell, rat RH35 whole cell, mouse RAW264.7 whole cell. IHC: human tonsil tissue.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Activated Histone Acetyltransferase p300/CBP-Related Signalling Pathways Mediate Up-Regulation of NADPH Oxidase, Inflammation, and Fibrosis in Diabetic Kidney.
Lazar AG, Vlad ML, Manea A, Simionescu M, Manea SA
Antioxidants (Basel, Switzerland) 2021 Aug 26;10(9)
Antioxidants (Basel, Switzerland) 2021 Aug 26;10(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NOX2 on paraffin-embedded human intestinal cancer tissue. Sample was incubated with NOX2 polyclonal antibody (Product# PA5-79118).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NOX2 in paraffin-embedded section of human tonsil tissue using NOX2 Polyclonal Antibody (Product # PA5-79118). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4°C. Peroxidase conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Pharmacological inhibition of histone acetyltransferase p300/CBP by C646 mitigates the glomerular expression of Nox subtypes in diabetic mice. ( A , C , E ) Quantification of fluorescence immunolabeling for glomerular Nox1, Nox2, and Nox4 subtypes. ( B , D , F ) Representative IF microscopy images taken at 40x magnification depicting the immunofluorescence staining (red) for Nox subtypes. Sections were counterstained with DAPI (blue) stain to detect the cell nuclei in the specimens. Note that the up-regulation of glomerular Nox1, Nox2, and Nox4 protein levels in diabetic mice is blunted following C646 treatment. n = 14-19/condition quantified glomeruli, *** p < 0.001, p -values taken in relation to non-diabetic + vehicle condition; ### p < 0.001, p -values taken in relation to diabetic + vehicle condition.
