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- Product number
- A00328-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-NOX2/gp91phox/CYBB Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for NOX2/gp91phox/CYBB detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Submitted references β-d-glucan from Antrodia Camphorata ameliorates LPS-induced inflammation and ROS production in human hepatocytes.
Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells.
Chen Q, Tang H, Zha Z, Yin H, Wang Y, Wang Y, Li H, Yue L
International journal of biological macromolecules 2017 Nov;104(Pt A):768-777
International journal of biological macromolecules 2017 Nov;104(Pt A):768-777
Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells.
Wang Y, Wang Y, Liu D, Wang W, Zhao H, Wang M, Yin H
Carbohydrate polymers 2015 Jul 10;125:135-45
Carbohydrate polymers 2015 Jul 10;125:135-45
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody (A00328-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human U937 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2/gp91phox/CYBB antigen affinity purified polyclonal antibody (Catalog # A00328-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX2/gp91phox/CYBB at approximately 65KD. The expected band size for NOX2/gp91phox/CYBB is at 65KD.
- Additional image
