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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ6611 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NOX2/gp91phox Antibody / CYBB
- Antibody type
- Polyclonal
- Description
- This highly specific NOX2/gp91phox antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NOX2/gp91phox antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) MCF7 and 2) Raji cell lysate with NOX2/gp91phox antibody. Predicted molecular weight ~65 kDa, can be observed at ~85 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U937 cells with NOX2/gp91phox antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX2/gp91phox antibody.
