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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-60810 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ILDR2 Recombinant Human Monoclonal Antibody (13G7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Human
- Isotype
- IgG
- Antibody clone number
- 13G7
- Vial size
- 100 µL
- Concentration
- 1.05 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ILDR2 in U251 cells. Samples were incubated with ILDR2 Recombinant Monoclonal antibody (Product # MA5-60810) using a dilution of 1:30 followed by FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ILDR2 in Hela cells. Samples were incubated with ILDR2 Recombinant Monoclonal antibody (Product # MA5-60810) using a dilution of 1:30 followed by FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ILDR2 in Jurkat cells. Samples were incubated with ILDR2 Recombinant Monoclonal antibody (Product # MA5-60810) using a dilution of 1:100 (red line) followed by FITC-conjugated Goat Anti-human IgG(H+L) at a 1:200 dilution for 35 min at 4°C. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1 µg/10^6 cells) for 45 min at 4°C. Control antibody (green line) was human IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
