RQ5671

antibody from NSJ Bioreagents

Targeting: RAB11B H-YPT3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5671

Provider

NSJ Bioreagents

Product name

RAB11B Antibody

Antibody type

Monoclonal

Description

This highly specific RAB11B antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

6C5

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the RAB11B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HEK293, 2) HeLa, 3) A549, 4) placenta, 5) HepG2, 6) Caco-2, 7) ThP-1 and 8) Raji lysate with RAB11B antibody. Predicted molecular weight ~24 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat 1) brain, 2) lung, 3) spleen, 4) C6 and mouse 5) brain, 6) lung, 7) spleen, 8) Neuro-2a and 9) RAW264.7 lysate with RAB11B antibody. Predicted molecular weight ~24 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human MCF7 cells with RAB11B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Caco-2 cells with RAB11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB11B antibody.