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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP21605SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21605SU-S, RRID:AB_10758448
- Product name
- anti BFAR / RNF47
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to amino acids 3-21 (EPQKSYVNTMDLERDEPLK) of human BAR
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of BAR in normal prostate and prostate carcinoma tissue lysates. 25 µg protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kDa. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using AP21605SU-S at 1/2000. Hematoxylin-eosin counterstain. Variable BAR expression is seen between patient samples.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using AP21605SU-S at 1/2000. A. Two cores from a human gliobastoma tissue microarray: 1 = fibrillary astrocytoma (grade I), and 2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-eosin counterstain.
