Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- AF2659 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nectin-4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse Nectin-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q96NY8
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Over-expression of Nectin-4 promotes progression of esophageal cancer and correlates with poor prognosis of the patients.
Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs.
A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development.
SLAM- and nectin-4-independent noncytolytic spread of canine distemper virus in astrocytes.
Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer.
Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer.
Nectin-4 mutations causing ectodermal dysplasia with syndactyly perturb the rac1 pathway and the kinetics of adherens junction formation.
Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008.
A role for PVRL4-driven cell-cell interactions in tumorigenesis.
Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
Deng H, Shi H, Chen L, Zhou Y, Jiang J
Cancer cell international 2019;19:106
Cancer cell international 2019;19:106
Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs.
Pratakpiriya W, Ping Teh AP, Radtanakatikanon A, Pirarat N, Thi Lan N, Takeda M, Techangamsuwan S, Yamaguchi R
Scientific reports 2017 Mar 23;7(1):349
Scientific reports 2017 Mar 23;7(1):349
A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development.
Kitayama M, Mizutani K, Maruoka M, Mandai K, Sakakibara S, Ueda Y, Komori T, Shimono Y, Takai Y
The Journal of biological chemistry 2016 Mar 11;291(11):5817-5831
The Journal of biological chemistry 2016 Mar 11;291(11):5817-5831
SLAM- and nectin-4-independent noncytolytic spread of canine distemper virus in astrocytes.
Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P
Journal of virology 2015 May;89(10):5724-33
Journal of virology 2015 May;89(10):5724-33
Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer.
Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y
Journal of experimental & clinical cancer research : CR 2015 Mar 28;34(1):30
Journal of experimental & clinical cancer research : CR 2015 Mar 28;34(1):30
Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer.
Galanis E, Atherton PJ, Maurer MJ, Knutson KL, Dowdy SC, Cliby WA, Haluska P Jr, Long HJ, Oberg A, Aderca I, Block MS, Bakkum-Gamez J, Federspiel MJ, Russell SJ, Kalli KR, Keeney G, Peng KW, Hartmann LC
Cancer research 2015 Jan 1;75(1):22-30
Cancer research 2015 Jan 1;75(1):22-30
Nectin-4 mutations causing ectodermal dysplasia with syndactyly perturb the rac1 pathway and the kinetics of adherens junction formation.
Fortugno P, Josselin E, Tsiakas K, Agolini E, Cestra G, Teson M, Santer R, Castiglia D, Novelli G, Dallapiccola B, Kurth I, Lopez M, Zambruno G, Brancati F
The Journal of investigative dermatology 2014 Aug;134(8):2146-2153
The Journal of investigative dermatology 2014 Aug;134(8):2146-2153
Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008.
Sakai K, Nagata N, Ami Y, Seki F, Suzaki Y, Iwata-Yoshikawa N, Suzuki T, Fukushi S, Mizutani T, Yoshikawa T, Otsuki N, Kurane I, Komase K, Yamaguchi R, Hasegawa H, Saijo M, Takeda M, Morikawa S
Journal of virology 2013 Jan;87(2):1105-14
Journal of virology 2013 Jan;87(2):1105-14
A role for PVRL4-driven cell-cell interactions in tumorigenesis.
Pavlova NN, Pallasch C, Elia AE, Braun CJ, Westbrook TF, Hemann M, Elledge SJ
eLife 2013 Apr 30;2:e00358
eLife 2013 Apr 30;2:e00358
Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
Noyce RS, Bondre DG, Ha MN, Lin LT, Sisson G, Tsao MS, Richardson CD
PLoS pathogens 2011 Aug;7(8):e1002240
PLoS pathogens 2011 Aug;7(8):e1002240
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Nectin-4 in Human Placenta. Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.