PA5-46164

antibody from Invitrogen Antibodies

Targeting: IFNE IFNE1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46164

Provider

Invitrogen Antibodies

Product name

IFN epsilon Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: IFSLFRANIS LDGWEENHTE KFLIQLHQQL EYLEALMGLE AEKLSGTLGS Sequence homology: Cow: 93%; Human: 100%; Pig: 79% PA5-46164 was successfully used to detect IFNE in endometrial tissue sections by IHC.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IFNE was performed on equine endometrial tissue sections. 5-µm-thick sections were prepared from formalin-fixed paraffin-embedded tissue blocks and were dried on slide plate at 37°C overnight. The sections were placed in a Bond Max Automated Immunohistochemistry Vision Biosystem (Leica Microsystems GmbH, Wetzlar, Germany) according to the following protocol. First, tissues were deparaffinized and pre-treated with Epitope Retrieval Solution 2 (Citrate based, pH 8) for 10 minutes. After washing steps, peroxidase blocking was carried out for 10 min using the Bond Polymer Refine Detection Kit DC9800 (Leica Microsystems GmbH). Tissues were again washed and then incubated with the primary antibody (1:200) for 30 min then the secondary antibody diluted 1:1000. Subsequently, tissues were incubated with polymer for 10 min and developed with DAB-Chromogen for 10 min. Data courtesy of Antibody Data Exchange Program.