LS-C820615

antibody from LSBio

Targeting: USH1C AIE-75, DFNB18, harmonin, NY-CO-37, NY-CO-38, PDZ-73, PDZ73, PDZD7C

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C820615

Provider

LSBio

Product name

Harmonin / USH1C Antibody (Preservative Free) LS-C820615

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

USH1C was immunoprecipitated using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate. 2 uL anti-USH1C rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-USH1C rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 75 kDa. Observed band size: 70 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

USH1C was immunoprecipitated using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate. 2 uL anti-USH1C rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-USH1C rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 75 kDa. Observed band size: 70 kDa.