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LearnLS-C820615
antibody from LSBio
Targeting: USH1C
AIE-75, DFNB18, harmonin, NY-CO-37, NY-CO-38, PDZ-73, PDZ73, PDZD7C
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C820615 - Provider product page
- Provider
- LSBio
- Product name
- Harmonin / USH1C Antibody (Preservative Free) LS-C820615
- Antibody type
- Polyclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-USH1C rabbit polyclonal antibody at 1:500 dilution. Lane A: Caco-2 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 73 kDa. Observed band size: 70 kDa. (We are unsure as to the identity of these extra bands.)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- USH1C was immunoprecipitated using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate. 2 uL anti-USH1C rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-USH1C rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 75 kDa. Observed band size: 70 kDa.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- USH1C was immunoprecipitated using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate. 2 uL anti-USH1C rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-USH1C rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 75 kDa. Observed band size: 70 kDa.
