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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [6]
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- Product number
- GTX84508 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84508, RRID:AB_10726952
- Product name
- CD32a antibody [20D1]
- Antibody type
- Monoclonal
- Reactivity
- Human, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 μg per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A .
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35μg) from 9 different cell lines by using anti-FCGR2A monoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP was performed using 500ul of DDDDK tagged FCGR2A overexpression lysates and 2ug of CD32a antibody. The precipitates were detected by DDDDK tag antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84508, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84508, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84508, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84508, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84508, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84508, Dilution 1:50)
