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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX84510 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84510, RRID:AB_10728397
- Product name
- CD32a antibody [6H7]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 μg per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A .
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP was performed using 500ul of DDDDK tagged FCGR2A overexpression lysates and 2ug of CD32a antibody. The precipitates were detected by DDDDK tag antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84510, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84510, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (GTX84510, Dilution 1:50)
