TA500651

antibody from OriGene

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500651

Provider

OriGene

Proper citation

OriGene Cat#TA500651, RRID:AB_10585418

Product name

Anti-FCGR2A (CD32) mouse monoclonal antibody, clone OTI16E7 (formerly 16E7)

Antibody type

Monoclonal

Description

Anti-FCGR2A (CD32) mouse monoclonal antibody, clone OTI16E7 (formerly 16E7)

Host

Mouse

Conjugate

Unconjugated

Epitope

FCGR2A

Isotype

IgG

Antibody clone number

OTI16E7

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A .

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-FCGR2A mouse monoclonal antibody (TA500651) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A (RC205786).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-FCGR2A mouse monoclonal antibody (TA500651).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500651, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500651, Dilution 1:50)

Validation comment

IHC